Elabscience

NCI-H82 [H82] Cell Line

Product Code:
CL-0712
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
41Y, Male
Background:
NCI-H82 [H82] is a morphological and biochemical variant of small cell lung cancer (SCLC), as the primary tumor from which this cell line derives does not exhibit classic SCLC pathological features. The cells express two canonical SCLC biomarkers, neuron-specific enolase (NSE) and brain-type creatine kinase isoenzyme (CK-BB), while showing undetectable expression of L-DOPA decarboxylase (DDC) and bombesin. Genetically, NCI-H82 [H82] produces an aberrantly sized 3.7 kb TP53 mRNA and harbors approximately 25-fold c-myc gene amplification, accompanied by a 24-fold elevation of c-myc transcript levels compared with normal cells. Functionally, the cell line expresses functional atrial natriuretic peptide (ANP) receptors, yet ANP treatment exerts no effect on its growth characteristics. Immunostaining confirms positive expression of neurofilaments and vimentin; additionally, NCI-H82 [H82] constitutively expresses mRNAs of v-fes, v-fms, H-ras, K-ras, N-ras, and c-raf-1.
Biosafety Level:
BSL-1
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Growth properties:
Suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like; suspended in clusters
Subcultivation Ratio:
1:2-1:4
Tissue:
Lung; derived from metastatic site: pleural effusion
Tumorigenic:
Yes; Yes, in nude mice.

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