Elabscience

SHP-77 Cell Line

Product Code:
CL-0718
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
54Y, Male
Antigen expression:
Blood Type O; Rh +; CD56; CD57 (HNK-1,Leu-7)
Background:
SHP-77 retains important characteristics of small cell lung cancer (SCLC) and possesses stable biochemical properties, allowing continuous cultivation. This cell line is an unusual undifferentiated large-cell variant of SCLC. Although it exhibits a variant morphology, it maintains the biochemical properties of classical SCLC. Electron microscopy reveals gland formation and the presence of intracytoplasmic lamellar bodies. These cells express the neuroendocrine marker L-dopa decarboxylase and contain dense-core secretory granules. SHP-77 can serve as a target for detecting cytotoxicity via 51Cr and 111In release in both in vitro assays and in vivo nude mouse models, and is used to evaluate the immune reactivity of cells and serum from lung cancer patients. This cell line can also be used to assess the immune status of SCLC patients undergoing radiotherapy or chemotherapy.
Biosafety Level:
BSL-1
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Gene expression:
neural cell adhesion molecule (NCAM) NKH-1
Growth properties:
Aggregated suspension with loose adherence
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Round
Subcultivation Ratio:
4-5x10^5 cells/mL
Tissue:
Lung
Tumorigenic:
Yes; Yes, the cells form tumors in athymic nude mice, and usually grow as circumscribed nodules without evidence of metastases.

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