Procell system

A-431-LUC Cell Line

Product Code:
CL-0723
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
85Y, Female
Background:
The A-431 cell line, established from an 85-year-old female patient, is one of a series of cell lines developed by D.J. Giard and colleagues. form rapidly growing subcutaneous tumors resembling malignant melanoma in anti-thymocyte globulin- and serum-treated NIH Swiss micess mice, form colonies on fibroblast feeder layers and in soft agar, and are a hypertriploid human cell line.
Biosafety Level:
BSL-2
Cell Type:
Cancer cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
DMEM [PM150210]+10% Nutrient+1% Supplement
Dissociation Duration:
5-6 min
Doubling Time:
~80-100 hours
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:3-1:6
Subcultivation Ratio:
1:3-1:6
Tissue:
Skin

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