Procell system

NCM460 Cell Line

Product Code:
CL-0749
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
68Y, Male
Background:
The NCM460 cell line is derived from normal human colonic mucosal epithelial cells and serves as a key in vitro model for investigating human intestinal physiology and pathology. This cell line was isolated from normal transverse colon tissue with no malignant lesions. NCM460 cells display typical gastrointestinal epithelial features, including the expression of epithelial markers such as villin and secretory components, which confirm their epithelial origin. Notably, the cells retain a non-tumorigenic phenotype, as they cannot form colonies in soft agar and fail to develop tumors in nude mice. NCM460 cells grow as a stable adherent monolayer under standard culture conditions. They are widely applied in studies on cell differentiation, drug transport, and epithelial barrier function. As a normal colonic epithelial cell model, NCM460 is commonly used to compare biological differences between normal and cancerous intestinal epithelial cells. It also provides a reliable platform to evaluate the impacts of dietary factors, drugs, and other exogenous stimuli on colonic epithelial homeostasis and lesions. Overall, this cell line is a powerful research tool for exploring gastrointestinal biology at cellular and molecular levels.
Biosafety Level:
BSL-1
Cell Type:
spontaneously immortalized cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Doubling Time:
~48 hours
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2-1:4
Tissue:
Colon; Mucosa
Tumorigenic:
No, tested in nude mice and athymic mice

Documents

References

  • https://www.tandfonline.com/doi/abs/10.1080/19490976.2024.2377567
  • https://www.sciencedirect.com/science/article/pii/S2590006425011986
  • https://pubs.acs.org/doi/abs/10.1021/acsami.5c10365