Elabscience

OE19 Cell Line

Product Code:
CL-0754
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
72Y, Male
Background:
The OE19 cell line, also known as JROECL19, was originally one of five permanent human esophageal adenocarcinoma (EAC) cell lines established in 1993. It was derived from a stage III tumor of a 72-year-old male patient and deposited in ECACC by Drs J.C. Rockett and A. Morris (Department of Biological Sciences, University of Warwick) and Dr S.J. Darnton (Birmingham Heartlands Hospital). Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines, but rather the large cell lung cancer cell line H460, the colorectal adenocarcinoma cell line SW620, and the gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs. All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC.
Biosafety Level:
BSL-1
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Dissociation Duration:
3-5 min
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:2-1:4
Tissue:
Esophagus

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