Procell system

HTR-8-Svneo Cell Line

Product Code:
CL-0765
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
6-12FW
Antigen expression:
cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha
Background:
The HTR-8-Svneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen.
Biosafety Level:
BSL-1
Cell Type:
Transformed cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:3
Subcultivation Ratio:
1:2-1:3
Tissue:
Placenta
Tumorigenic:
No (tested in soft agar and nude mice).

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S2090123225009178
  • https://www.sciencedirect.com/science/article/pii/S1385894725081616
  • https://link.springer.com/article/10.1186/s12967-026-08513-3