Procell system

WSU-DLCL2 Cell Line

Product Code:
CL-0774
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
41Y, Male
Background:
WSU-DLCL2 was established in 1990 from pleural effusion collected from a 41-year-old male patient with relapsed/progressive B-cell non-Hodgkin lymphoma (B-NHL). The lymphoma was classified as intermediate-grade non-cleaved diffuse large cell lymphoma, transformed from low-grade follicular small cleaved cell lymphoma. WSU-DLCL2 harbors the EZH2 Y641F mutation, and corresponding exome and RNA sequencing datasets are publicly available for this cell line.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Doubling Time:
~18 hours (PubMed=9607591); ~50-70 hours (DSMZ=ACC-575)
Growth Properties:
Suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Round and oval cells, growing singly or in small suspension clusters
Split Ratio:
1:3-1:5
Subcultivation Ratio:
1:3-1:5
Tissue:
Pleural effusion

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0041008X24003478
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/kjm2.70158