Elabscience

Mino Cell Line

Product Code:
CL-0779
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

No additional charges, what you see is what you pay! *

Show prices   How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Age Gender:
64Y, Male
Antigen expression:
CD3-, CD5+, CD10-, CD19+, CD20+, CD23- (verified at ATCC)
Background:
The Mino cell line consists of lymphoblasts isolated from the peripheral blood of a 64-year-old White male with mantle cell lymphoma (MCL). This cell line can be used for immunology research. The cells are large, growing singly and in small clumps in vitro. The immunophenotype determined by flow cytometry is compatible with MCL. Western blotting shows expression of cyclin D1 but no detectable cyclin D2 or cyclin D3; the retinoblastoma protein is predominantly phosphorylated. The cells express tumor suppressor gene products including p53, p16 (INK4a), and p21 (WAF1).
Biosafety Level:
BSL-1
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+20% Nutrient+1% Supplement
Gene expression:
cyclin D2; cyclin D3; p53; p16 (INK4a); p21(WAF1)
Growth properties:
Suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Lymphoblast-like
Subcultivation Ratio:
1:2-1:3
Tissue:
Peripheral blood

Documents