Procell system

LN-18 Cell Line

Product Code:
CL-0846
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
65Y, Male
Antigen expression:
HLA A2, A9, B5, BW35, DRW3
Background:
LN-18 is a cell line with an epithelial-like morphology, isolated in 1976 from the right temporal lobe of a 65-year-old Caucasian male patient with glioblastoma. The cells are negative for glial fibrillary acidic protein (GFAP) and S100 protein. These cells harbor a mutated TP53 gene and possible homozygous deletions of the p16 and p14ARF tumor suppressor genes. They carry wild-type PTEN. Stimulation with Fas ligand induces apoptotic cell death within 16 hours. The cells are also killed by puromycin in a dose-dependent manner. Bcl-2 protects these cells from Fas ligand?induced cell death but has only a minor protective effect against puromycin-induced apoptosis.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
DMEM [PM150210]+5% Nutrient+1% Supplement
Dissociation Duration:
3-5 min
Doubling Time:
~72 hours (PubMed=7211194); ~33 hours (PubMed=9842975)
Gene expression:
fibronectin
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
01:02
Subcultivation Ratio:
1~2
Tissue:
Brain
Tumorigenic:
Yes; Yes, forms tumors in nude mice.

Documents

References

  • https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202514191
  • https://www.sciencedirect.com/science/article/pii/S0898656824004881
  • https://www.sciencedirect.com/science/article/pii/S0006899326000892