Elabscience

NCI-H526 Cell Line

Product Code:
CL-0873
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
55Y, Male
Background:
NCI-H526 is an epithelial cell line that was isolated from the lungs of a 55-year-old, White male with carcinoma. NCI-H526 expresses elevated levels of 2 biochemical markers of SCLC: neuron specific enolase and the brain isoenzyme of creatine kinase. They do not express L-dopa carboxylase or bombesin-like immunoreactivity. These cells express the c-kit gene as well as the N-myc gene, but not c-myc or L-myc. N-myc is amplified and p75 c-myb expression was observed. NCI-H526 also expresses the proto-oncogenes N-ras, Ki-ras, Ha-ras, and c-raf1. Only trace amounts of the retinoblastoma susceptibility gene, RB mRNA were detected. RB protein was not detected. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. Abnormally sized mRNA was present.
Biosafety Level:
BSL-1
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Growth properties:
Suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Round; growing in clusters
Subcultivation Ratio:
1:3-1:6
Tissue:
Lung
Tumorigenic:
Yes; Yes, the cells produce tumors in athymic nude mice

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