Procell system

HLE-B3 Cell Line

Product Code:
CL-0913
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
5-12M, Female
Background:
The HLE-B3 cell line was derived from a human lens obtained within 24 hrs from 5-12 month old patient who underwent treatment for retinopathy of prematurity. At 60% confluence, cells at passage 3 were infected with Ad12-SV40.
Biosafety Level:
BSL-2
Cell Type:
Transformed cell line
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Doubling Time:
~72 hours
Gene expression:
beta crystallin; gamma crystallin
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:3
Subcultivation Ratio:
1:2-1:3
Tissue:
Eye; Ocular lens

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S014181302509659X
  • https://www.sciencedirect.com/science/article/pii/S1046202326000484
  • https://www.tandfonline.com/doi/abs/10.1080/10715762.2025.2475390