Elabscience

WM115 Cell Line

Product Code:
CL-0914
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
55Y, Female
Background:
WM115 cells are a tumorigenic (VGP) primary melanoma cell line with metastatic potential. This cell line was established from a metastatic site (right anterior leg skin) in a 55-year-old female with superficial spreading melanoma. The subject had a VGP tumor with Clark level III and a thickness of 2.24 mm. This cell line harbors a specific V600D (Val600Asp) mutation at codon 600 of the BRAF gene. It also exhibits PTEN loss of function, including hemizygous deletion. This cell line is derived from the same patient as the cell lines WM239A, WM165-1, and WM266-4. WM115 originated from a skin metastasis, while WM239A, WM165-1, and WM266-4 were derived from individual lymph-node metastases. WM115 is wild-type for the N-RAS, c-KIT, and CDK4 genes. When injected into immunocompromised mice, WM115 cells form xenograft tumors.
Biosafety Level:
BSL-1
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
DMEM [PM150210]+10% Nutrient+1% Supplement
Dissociation Duration:
5-7 min
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Fibroblast-Like
Subcultivation Ratio:
1:2-1:4
Tissue:
Skin; derived from metastatic site: thigh

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