Elabscience

hTERT-HPNE Cell Line

Product Code:
CL-0921
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
52Y, Male
Background:
The hTERT-HPNE cell line was established from pancreatic tissue of a 52-year-old male patient and immortalized by transduction with the retroviral expression vector pBABEpuro carrying the hTERT gene. Following transduction, the cells exhibited positive telomerase activity, did not undergo senescence, and continued to proliferate beyond 150 population doublings. hTERT-HPNE cells display features characteristic of intermediate cells formed during acinar-to-ductal metaplasia, including an undifferentiated phenotype, expression of nestin, and evidence of active Notch signaling.
Biosafety Level:
BSL-2
Cryopreservation:
90% FBS+10% DMSO
Culture medium:
DMEM [PM150210]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:2-1:4
Tissue:
Pancreas; Pancreatic duct

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