Elabscience

HT-29-GFP Cell Line

Product Code:
CL-0949
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
44Y, Female
Background:
HT-29 cells were established in 1964 by J. Fogh from a primary tumor via explant culture in F12 medium supplemented with 15% FBS. The cell line is currently maintained in McCoy's 5A medium with the same serum concentration. HT-29 cells are tumorigenic in both nude mice and steroid-treated hamsters, and synthesize IgA, CEA, TGF-β-binding proteins, and mucins. The HT-29-GFP cell line was constructed from HT-29 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for assays such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 2.0 ug/mL.
Biosafety Level:
BSL-2
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
McCoy's 5A [PM150710]+10% Nutrient+1% Supplement
Dissociation Duration:
3-5 min
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:3-1:6
Tissue:
Colon
Tumorigenic:
Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters.

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