Procell system

WM-266-4 Cell Line

Product Code:
CL-0950
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
54Y, Female
Background:
WM266-4 is a metastatic human melanoma cell line with a small, flat mesenchymal morphology. This cell line was derived from the same patient as the cell lines WM115, WM239A, and WM165-1. The WM115 cell line originated from the primary tumor, whereas WM266-4, WM165-1, and WM239A were derived from individual lymph node metastases. The subject (a 55-year-old female) displayed a vertical growth phase (VGP) melanoma with a Clark level III tumor measuring 2.24 mm in thickness. This cell line harbors the specific V600D (Val600Asp) mutation at codon 600 of the BRAF gene and also exhibits loss of PTEN function, including a hemizygous deletion. The WM266-4 cell line is wild-type for the N-RAS, c-KIT, and CDK4 genes. When injected into immunocompromised mice, WM266-4 cells form xenograft tumors.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Dissociation Duration:
2-4 min
Doubling Time:
~30 hours
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2-1:4
Tissue:
Skin

Documents

References

https://biolres.biomedcentral.com/articles/10.1186/s40659-017-0141-8