Elabscience

A549-GFP Cell Line

Product Code:
CL-0975
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
58Y, Male
Background:
This cell line was established in 1972 by D.J. Giard via explant culture of lung carcinoma tissue derived from a 58-year-old Caucasian male. A549 cells can synthesize lecithin containing a high proportion of unsaturated fatty acids via the cytidine diphosphocholine pathway and are keratin-positive. The A549-GFP cell line was constructed from A549 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for experiments such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 7.0 ug/mL.
Biosafety Level:
BSL-2
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
Ham's F-12K [PM150910]+10% Nutrient+1% Supplement1
Dissociation Duration:
2-3 min
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:3-1:6
Tissue:
Lung

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