Elabscience

PC-3-GFP Cell Line

Product Code:
CL-1009
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
62Y, Male
Background:
PC-3 was isolated from a bone metastatic lesion of grade IV prostatic adenocarcinoma resected from a 62-year-old Caucasian male patient. The cell line displays low acid phosphatase and 5α-reductase enzymatic activity. For drug screening: Fluorescent gene fragments are integrated into the cellular genome through lentiviral transduction, driving intracellular fluorescent protein expression that can be visualized by fluorescence microscopy. This labeling permits convenient tracking and detection of target cells. Fluorescent protein expression intensity may differ across cells due to variable lentiviral integration efficiency. Antibiotic screening can be applied to enrich populations with robust fluorescence. For routine maintenance, periodic puromycin selection at a final concentration of 4 ug/mL is suggested (2?3 times monthly or at user-defined intervals). An additional single round of puromycin selection is required immediately post-thaw of cryopreserved stocks. Daily puromycin supplementation is not needed during regular cultivation.
Biosafety Level:
BSL-1
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
Ham's F-12K [PM150910]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Growth properties:
Semi-adherent and semi-suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:3-1:6
Tissue:
Prostate; derived from metastatic site: bone

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