Elabscience

PC-3-RFP Cell Line

Product Code:
CL-1010
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
62Y, Male
Background:
PC-3 was isolated from a bone metastatic lesion of grade IV prostatic adenocarcinoma resected from a 62-year-old Caucasian male patient. The cell line displays low acid phosphatase and 5α-reductase enzymatic activity. For drug screening: Fluorescent gene fragments are integrated into the cellular genome via lentiviral transduction, enabling the cells to express fluorescent proteins observable under a fluorescence microscope and allowing convenient tracking and detection of labeled cells. Variable fluorescent protein expression may occur due to the characteristics of lentiviral integration. Antibiotic selection can be performed to improve fluorescent expression levels. For routine cell culture, periodic supplemental selection with puromycin at a final concentration of 4 ug/mL is recommended, with a frequency of two to three times per month or as customized. One round of puromycin selection is additionally required after thawing cryopreserved cell stocks. Daily puromycin supplementation is not necessary during routine cultivation.
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
Ham's F-12K [PM150910]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Growth properties:
Semi-adherent and semi-suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:3-1:6
Tissue:
Prostate; derived from metastatic site: bone

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