Elabscience

MCF-7-FLUC Cell Line

Product Code:
CL-1016
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
69Y, Female
Antigen expression:
Blood Type O; Rh+
Background:
The MCF-7 cell line was isolated from the pleural effusion of a 69-year-old Caucasian female patient with breast cancer. The cells retain several characteristics of differentiated mammary epithelium, including the ability to respond to estradiol via cytoplasmic estrogen receptors and the capacity to form domes. They also express the WNT7B oncogene. Tumor necrosis factor-alpha (TNF-α) can inhibit the growth of MCF-7 cells, and anti-estrogen treatment modulates the secretion of insulin-like growth factor binding proteins (IGFBPs). The MCF-7-FLUC cell line was constructed from MCF-7 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level firefly luciferase expression. This cell line exhibits low passage, high viability, and good condition. It can be used as a positive control in firefly luciferase activity assays and for in vivo animal imaging experiments. Selection drug concentration: Puro = 2.0 ug/mL.
Biosafety Level:
BSL-2
Cryopreservation:
90% FBS+10% DMSO
Culture medium:
MEM, with NEAA [PM150410]+10 ug/mL Insulin [PB180432]+10% Nutrient+1% Supplement
Dissociation Duration:
4-5 min
Gene expression:
insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Receptor expression:
estrogen receptor, expressed
Subcultivation Ratio:
1:2-1:3
Tissue:
Mammary gland/breast; derived from metastatic site: pleural effusion

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