Elabscience

WIL2 S Cell Line

Product Code:
CL-1039
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
5Y, Male
Background:
WIL2-S is a human B lymphocyte cell line isolated from the spleen of a 5-year-old White male patient with hereditary spherocytosis. As a HAT-sensitive variant of the parental WIL2 human B-cell line, it serves as a suitable host for transfection experiments.
Biosafety Level:
BSL-2
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Growth properties:
Aggregated suspension with loose adherence
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Lymphoblast-like
Subcultivation Ratio:
1:2-1:3
Subculturing:
1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 ? g (approximately 250 ? g). 2. Adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio.
Tissue:
Spleen

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