Elabscience

U-2 OS-LUC-EGFP Cell Line

Product Code:
CL-1058
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
15Y, Female
Antigen expression:
Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
Background:
The U-2 OS cell line (originally named 2T) was established in 1964 by J. Pont?n and E. Saksela from a moderately differentiated sarcoma of the tibia in a 15-year-old female. When co-cultured with WI-38 cells or tested by complement fixation (CF) assay for simian virus 40 (SV40), respiratory syncytial virus (RSV), or adenoviruses, no viruses were detected. U-2 OS cells express insulin-like growth factor I (IGF-I) and II (IGF-II) receptors, as well as osteosarcoma-derived growth factor (ODGF). U-2 OS-LUC-EGFP is a derivative cell line generated by infecting parental U-2 OS cells with a recombinant lentivirus carrying luciferase and EGFP genes. This cell line stably expresses both luciferase and enhanced green fluorescent protein (EGFP), enabling its application in various in vivo and in vitro experiments requiring cell tracking. Selection drug concentration: Puro = 2.0 ug/mL.
Biosafety Level:
BSL-2
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
McCoy's 5A [PM150710]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Gene expression:
osteosacoma derived growth factor (ODGF), Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Epithelial-like
Receptor expression:
insulin-like growth factor I (IGF-I);insulin-like growth factor II (IGF II)
Subcultivation Ratio:
1:3-1:4
Subculturing:
The U-2 OS cell line (originally named 2T) was established in 1964 by J. Pont?n and E. Saksela from a moderately differentiated sarcoma of the tibia in a 15-year-old female. When co-cultured with WI-38 cells or tested by complement fixation (CF) assay for simian virus 40 (SV40), respiratory syncytial virus (RSV), or adenoviruses, no viruses were detected. U-2 OS cells express insulin-like growth factor I (IGF-I) and II (IGF-II) receptors, as well as osteosarcoma-derived growth factor (ODGF). U-2 OS-LUC-EGFP is a derivative cell line generated by infecting parental U-2 OS cells with a recombinant lentivirus carrying luciferase and EGFP genes. This cell line stably expresses both luciferase and enhanced green fluorescent protein (EGFP), enabling its application in various in vivo and in vitro experiments requiring cell tracking. Selection drug concentration: Puro = 2.0 ug/mL.
Tissue:
Bone

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