Elabscience

K-562-GFP Cell Line

Product Code:
CL-1071
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
53Y, Female
Background:
The continuous cell line K-562 was established by Lozzio from the pleural effusion of a terminally ill 53-year-old female patient with chronic myelogenous leukemia. K-562 cells were originally classified as highly undifferentiated granulocytic cells; however, studies by Anderson et al. on their surface membrane properties identified K-562 as a human erythroleukemia cell line. K-562 cells are widely used as highly sensitive in vitro targets in natural killer (NK) cell assays. Pross et al. employed K-562 cells for the detailed in vitro detection of NK cells, including the mathematical quantification of NK cell activity. K-562 blasts are pluripotent hematopoietic malignant cells capable of spontaneous differentiation into recognizable erythroid progenitors, granulocytes, monocytes, and other lineages. K-562 cells are EBNA-negative. The K-562-GFP cell line was constructed from K-562 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for assays such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 7.0 ug/mL.
Biosafety Level:
BSL-2
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
IMDM [PM150510]+10% Nutrient+1% Supplement
Growth properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Lymphoblast-like
Subcultivation Ratio:
3x10^5-5x10^5 cells/mL
Subculturing:
Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 ? g) for 3 minutes.
Tissue:
Bone Marrow

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