Elabscience

MCF7-LUC-EGFP Cell Line

Product Code:
CL-1074
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
69Y, Female
Antigen expression:
Blood Type O; Rh+
Background:
The MCF-7 cell line was isolated from the pleural effusion of a 69-year-old Caucasian female patient with breast cancer. The cells retain several characteristics of differentiated mammary epithelium, including the ability to respond to estradiol via cytoplasmic estrogen receptors and the capacity to form domes. They also express the WNT7B oncogene. Tumor necrosis factor-alpha (TNF-α) can inhibit the growth of MCF-7 cells, and anti-estrogen treatment modulates the secretion of insulin-like growth factor binding proteins (IGFBPs). The MCF-7-LUC-EGFP cell line was constructed from MCF-7 cells via infection with a recombinant lentivirus carrying the luciferase and EGFP genes, generating a stable cell line that constitutively expresses both luciferase and enhanced green fluorescent protein (EGFP). This cell line is suitable for various in vivo and in vitro experiments requiring cell tracking. Selection drug concentration: Puro = 2.0 ug/mL.
Biosafety Level:
BSL-2
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
MEM, with NEAA [PM150410]+10 ug/mL Insulin [PB180432]+10% Nutrient+1% Supplement
Dissociation Duration:
3-5 min
Gene expression:
insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Epithelial-like
Receptor expression:
estrogen receptor, expressed
Subcultivation Ratio:
1:3-1:4
Subculturing:
1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ?g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Tissue:
Mammary gland/breast; derived from metastatic site: pleural effusion

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