HCT 116-GFP Cell Line

Male

HCT 116 cells are one of three malignant cell lines isolated by M. Brattain et al. in 1979 from a male patient with colon carcinoma. HCT 116 cells form colonies in semisolid agarose medium and are tumorigenic in athymic nude mice, producing tumor nodules. The HCT 116-GFP cell line was constructed from HCT 116 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for assays such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 2.0 ug/mL.

BSL-2

Freezing Medium (Serum-free & animal origin-free) [PB180438]

McCoy's 5A [PM150710]+10% Nutrient+1% Supplement

1-2 min

carcinoembryonic antigen (CEA) 1 ng per 1x10^6 cells per 10 days.

Adherent

Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C

2 to 3 times per week

Epithelial-like

1:3-1:6

1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ?g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.

Colon