Elabscience

U-937-GFP Cell Line

Product Code:
CL-1091
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
37Y, Male
Background:
The U-937 cell line was established by Sundstr?m and Nilsson in 1974 from pleural effusion of a patient with histiocytic lymphoma. Studies have shown that U-937 cells can be induced to undergo terminal monocytic differentiation by human mixed lymphocyte culture supernatant, phorbol ester, vitamin D3, γ-interferon, tumor necrosis factor, and retinoic acid. U-937 cells are negative for immunoglobulin production and EBV expression. The cells express Fas antigen and are sensitive to TNF and anti-Fas antibodies. The U-937-GFP cell line was constructed from U-937 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for experiments such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 2.0 ug/mL.
Biosafety Level:
BSL-2
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Growth properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Monocytic
Subcultivation Ratio:
5x10^5-1x10^6 cells/mL
Subculturing:
Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 ? g) for 3 minutes.
Tissue:
Pleural effusion

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