Elabscience

GM23255 Cell Line

Product Code:
CL-1119
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
16Y, Male
Background:
GM23255 is an immortalized cell line obtained by immortalizing B lymphocytes with Epstein-Barr virus (EBV). It was derived from a 16-year-old Asian male patient with spinal muscular atrophy (SMA). The donor has 0 copies of the SMN1 gene and 3 copies of the SMN2 gene.
Biosafety Level:
BSL-2
Cryopreservation:
90% FBS+10% DMSO
Culture medium:
RPMI-1640 [PM150110]+15% Nutrient+1% Supplement
Growth properties:
Suspension with clustering
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Lymphoblast-like; suspension growth with cell clumps
Subcultivation Ratio:
1:2-1:3
Subculturing:
Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 ? g) for 3 minutes.
Tissue:
Peripheral blood

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