Elabscience

TOV-21G Cell Line

Product Code:
CL-1145
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

No additional charges, what you see is what you pay! *

Show prices   How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Age Gender:
62Y, Female
Background:
TOV-21G was derived from a 62-year-old French-Canadian female patient with primary ovarian adenocarcinoma and no family history of ovarian cancer. This cell line was established in October 1991 from ovarian tissue and exhibits an epithelial morphology. TOV-21G is widely used in ovarian cancer research, particularly in studies on TRAIL resistance. Subtoxic doses of tanshinone IIA have been proven to enhance TRAIL-induced apoptosis in TOV-21G cells. The combination of tanshinone IIA and TRAIL is therefore being explored as a promising therapeutic strategy for ovarian cancer.
Biosafety Level:
BSL-1
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
Complete culture medium for TOV-21G
Dissociation Duration:
3-4 min
Gene expression:
keratin
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Epithelial-like
Subcultivation Ratio:
1:2-1:4
Subculturing:
1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ?g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Tissue:
Ovary
Tumorigenic:
Yes; Yes, form colonies in soft agar Yes, the cells are tumorigenic in nude mice

Documents