Elabscience

UPCI-SCC-154 Cell Line

Product Code:
CL-1149
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
54Y, Male
Background:
UPCI-SCC-154 is a squamous cell carcinoma cell line isolated in 1996 from the tongue of a 54-year-old White male patient with tongue squamous cell carcinoma. The cells grow in islands and the monolayer does not reach full confluence. This cell line is positive for human papillomavirus (HPV), a key etiologic factor for oropharyngeal squamous cell carcinoma, making it widely used in related research. Sequencing of TP53 exons 5?8 confirmed the absence of TP53 mutations, and FISH analysis detected no amplification of the 11q13 chromosomal band. Deposited by S. Gollin, UPCI-SCC-154 is suitable for investigating oral cancer onset, progression, prognosis, and corresponding therapeutic interventions.
Biosafety Level:
BSL-2
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
DMEM [PM150210]+10% Nutrient+1% Supplement
Dissociation Duration:
3-5 min
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Epithelial-like
Subcultivation Ratio:
1:2-1:3
Subculturing:
1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ?g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Tissue:
Oral cavity; Larynx

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