Elabscience

NCI-BL1437 Cell Line

Product Code:
CL-1169
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
60Y, Male
Background:
NCI-BL1437 is an Epstein?Barr virus (EBV)-transformed B lymphoblastoid cell line established from the peripheral blood of a 60-year-old Caucasian male. NCI-H1437, a lung adenocarcinoma cell line, was derived from the same patient.
Biosafety Level:
BSL-2
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
DMEM/F12 [PM150312]+0.5% ITS-G [PB180429]+10 nM Supplement1+10 nM Supplement2+10% Nutrient+1% Supplement3
Growth properties:
Suspension with clustering
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Lymphoblast-like
Subcultivation Ratio:
1:2-1:3
Subculturing:
Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 ? g) for 3 minutes.
Tissue:
Peripheral blood

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