Elabscience

RL Cell Line

Product Code:
CL-1190
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
52Y, Male
Antigen expression:
CD19 +; CD20 +;CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
Background:
The RL cell line is a human non-Hodgkin B-cell lymphoma cell line established in 1983 from the ascites of a 52-year-old male patient with diffuse large B-cell lymphoma. Treatment with protein kinase C-activating phorbol esters, including PMA and PdBu, results in significant growth inhibition in RL cells. This cell line is negative for the Epstein?Barr virus (EBV) genome.
Biosafety Level:
BSL-1
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Gene expression:
immunoglobulin, surface IgM; and IgD; secreted IgM (low levels)
Growth properties:
Suspension with multicellular aggregation
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Lymphoblast-like
Subcultivation Ratio:
1:2-1:4
Subculturing:
Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 ? g) for 3 minutes.
Tissue:
Ascites

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