Small RNA Marker

The Small RNA Marker is not prepared for estimating of RNA amount. RNA is very sensitive to degradation by nucleases. To avoid damaging the Small RNA Marker , use extreme care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the Small RNA Marker on ice and keep it on ice while using.

The Small RNA Marker consists of five single-stranded RNAs (ssRNA). The 20, 30, 40 and 50 bases RNAs are synthesized by chemically (non phosphorylated). The 100 bases RNA is synthesized by in vitro transcription. The Small RNA Marker is suitable for determinating size of small-size ssRNA in denaturing polyacrylamide gel electrophoresis. The Small RNA Marker can be visualized by ethidium bromide staining.

After 18 hrs incubation of the Small RNA Marker at 37°C, no visible degradation of the marker is observed in 12.5 % polyacrylamide / 7.5 M urea gel electrophoresis.

1 uL/lane

10 mM Tris-HCl (pH 8.0) buffer containing 1 mM EDTA (Ammonium acetate is slightly contained)