Abnova Corporation

E. coli Poly(A) Polymerase

Product Code:
DM0015
Regulatory Status:
GMP grade
Application:
In vitro transcription (IVT)
Storage:
Store at -20C.Aliquot to avoid repeated freezing and thawing.
 

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This product comes from: Taiwan.
Typical lead time: 14-21 working days.
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Further Information

Description:
E. coli Poly (A) Polymerase does not depend on the presence of template, and can catalyze the sequential incorporation of ATP in the form of AMP into the 3' terminal of RNA, i.e., the addition of polyA tail to the 3' terminal of RNA.
Form:
Liquid
Recommended Dilutions:
For RNA 3' end labeling.Poly(A) tails are added to RNA for cloning or affinity purification. For example, Poly (A) was added to miRNA to provide oligo-dT primer binding sites for cDNA synthesis.Improve the efficiency of RNA translation in eukaryot
Storage Buffer:
20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.1%TritonX-100, 50% (v/v) Glycerol, pH 8.0.