Human Macrophage Migration Inhibitory Factor/MIF Recombinant Protein (86-033)

Product Code:
PSI-86-033

Supplier:
ProSci

Regulatory Status:
RUO

Target Species:
Human

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunogen
SDS-PAGE
Western Blot (WB)

Shipping:
RT

Storage:
Store at -20°C. Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

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Further Information

Application Note:

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test

Background:

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era . Regardless, it is one of the least understood of all inflammatory mediators . Secretion occurs nonclassically via an ABCA1 transporter. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer . Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1. Amino acids 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity.

Buffer:

Lyophilized from a 0.22 um filtered solution of 20mM PB,150mM NaCl,pH7.4/20mM PB,150mM NaCl,pH7.4,50% glycerol

NCBI Gene ID:

4282

Physical State:

Lyophilized

Predicted MW:

13.32 kDa

Protein Accession:

NP_002406.1

Source Species:

E. coli

SwissProt:

P14174

Documents

Datasheet