Pseudouridine / 5-ribosyluracil Rabbit Recombinant Antibody (81-854)

DB,1:1000 - 1:10000|ELISA,Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements.

Pseudouridine (ψ) was among the first post-transcriptional modifications discovered and is overall one of the most abundant (1). It is present in a wide range of cellular RNAs and is highly conserved across species. ψ is derived from uridine (U) via base-specific isomerization catalyzed by ψ synthases. The site-specific pseudouridylation goes through either snoRNA-dependent (requires H/ACA RNP) or -independent mechanism (requires pseudouridine synthase (PUS) family enzymes)(2). It has an extra hydrogen-bond donor at its non-Watson-Crick edge. When incorporated into RNA, ψ can alter RNA secondary structure by increasing base stacking, improving base pairing and rigidifying sugar-phosphate backbone5. The chemical and physical properties of RNA can be altered with the incorporation of ψ, which could contribute to subsequent cellular functions.

Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Batch dependent

Unconjugated

CAS: 1445-07-4

Pseudouridine

Liquid