Rabbit anti GFP-Tag Recombinant Antibody (84-520)

WB,1:5000 - 1:20000|IF/ICC,1:50 - 1:200|IP,0.5ug-4ug antibody for 100ug-400ug extracts of whole cells|ELISA,Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements.|CHIP,5ug antibody for 10ug-15ug of Chromatin

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.

Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Batch dependent

Unconjugated

GFP

Liquid

27kDa

P42212

P42212