Schistosoma Japonicum GST-His Recombinant Protein (86-070)

Product Code:
PSI-86-070

Supplier:
ProSci

Regulatory Status:
RUO

Target Species:
Flatworm

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunogen
SDS-PAGE
Western Blot (WB)

Shipping:
RT

Storage:
Store at -20°C. Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

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Further Information

Application Note:

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test

Background:

Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 22amino acids(roughly 26 KDa), which, compared to other tags like the Myc-or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include athrombindomain for cleavage of the GST tag during protein purification.

Buffer:

Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.

Physical State:

Lyophilized

Predicted MW:

26.34 kDa

Source Species:

E. coli

SwissProt:

P08515

Documents

Datasheet