Abnova Corporation

IgE (Human) ELISA Kit

Product Code:
KA8352
Product Group:
ELISA Kits
Regulatory Status:
RUO
Target Species:
Human
Application:
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Store the kit at 4C. Don't cryopreserve.
 

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This product comes from: Taiwan.
Typical lead time: 14-21 working days.
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Further Information

Description:
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IgE antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IgE bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB is catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IgE in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.