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Further Information
Description:
Choline is an essential nutrient and plays a key role in many biological processes. Choline and its metabolites are needed for various physiological purposes, including: structural integrity of cell membranes, cell membrane signaling, cholinergic neurotransmission, and additionally, they are a major source of methyl groups that are needed for the biosynthesis of lipids and the regulation of metabolic pathways. Choline deficiency plays a role in liver disease, atherosclerosis, neurological disorders, and organ dysfunction. This colorimetric assay is based on choline oxidase-catalyzed oxidation of free choline, where the formed hydrogen peroxide is then catalyzed by peroxidase and reacts with 4-aminoantipyrine to form a colored product that can be read at 550nm. The intensity of the colored reaction product is directly proportional to the concentration of choline in the sample.
Extra Description:
The Choline Assay kit measures choline level in various types of samples, including cells, serum, plasma, and tissue. This product is for research purposes only and is not approved for use in animals, humans, or diagnostic procedures.
