ProSci

SARS Spike Antibody

Product Code:
PSI-3219
Product Group:
Primary Antibodies
Supplier:
ProSci
Host Type:
Rabbit
Antibody Isotype:
IgG
Antibody Clonality:
Polyclonal
Regulatory Status:
RUO
Application:
Enzyme-Linked Immunosorbent Assay (ELISA)
Product Type:
Microbial and Virus Specific
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<strong>Figure 1 ELISA Test </strong><br> Antibodies: SARS-CoV Spike Antibody, 3219 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 ng/mL to 1000ng/mL.
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<strong>Figure 1 ELISA Test </strong><br> Antibodies: SARS-CoV Spike Antibody, 3219 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 ng/mL to 1000ng/mL.

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Further Information

Additional Names:
SARS-CoV Spike Antibody: E2, Spike glycoprotein, E2, S glycoprotein
Application Note:
SARS-CoV Spike antibody can be used for the detection of SARS-CoV Spike protein in ELISA. It will detect 5 ng of free peptide at 1 μg/mL.
Background:
SARS-CoV Spike Antibody: A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
Background References:
  • Marra et al. Science 2003;300:1399-404.
  • Rota et al. Science 2003;300:1394-9.
  • Navas-Nartin et al. J Neurovirol. 2004;10:75-85.
  • Li et al. Nature 2003;426:450-4.
Buffer:
SARS-CoV Spike Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (14%)
Immunogen:
Anti-SARS-CoV Spike antibody (3219) was raised against a peptide corresponding to 14 amino acids near the amino of SARS-CoV Spike glycoprotein.

The immunogen is located within the first 50 amino acids of SARS-CoV Spike.
ISOFORMS:
SARS-CoV Spike has one isoform (1255aa).
NCBI Gene ID #:
1489668
NCBI Official Name:
Spike glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
SARS coronavirus
Physical State:
Liquid
Protein Accession #:
P59594
Protein GI Number:
30173397
Purification:
SARS-CoV Spike Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P59594
User NOte:
Optimal dilutions for each application to be determined by the researcher.