ProSci

Caspase-1 Antibody

Product Code:
PSI-3463
Product Group:
Primary Antibodies
Supplier:
ProSci
Host Type:
Rabbit
Antibody Isotype:
IgG
Antibody Clonality:
Polyclonal
Regulatory Status:
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Product Type:
Inflammasomes
1 / 3
Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.
2 / 3
Immunohistochemistry of caspase-1 in human spleen tissue with caspase-1 antibody at 10 μg/mL.
3 / 3
Immunofluorescence of Caspase-1 in Hela cells with Caspase-1 antibody at 20 μg/mL.
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Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunohistochemistry of caspase-1 in human spleen tissue with caspase-1 antibody at 10 μg/mL.
Immunofluorescence of Caspase-1 in Hela cells with Caspase-1 antibody at 20 μg/mL.

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Further Information

Additional Names:
Caspase-1 Antibody: ICE, P45, IL1BC, IL1BCE, Caspase-1, Interleukin-1 beta convertase, CASP-1
Application Note:
Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.
Background References:
  • Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. CCell 2004; 117:561-74.
  • Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
  • Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
  • Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
Buffer:
Caspase-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (80%), Horse: (73%)
Immunogen:
Caspase-1 antibody was raised against a 15 amino acid synthetic peptide from near the middle of human Caspase-1.

The immunogen is located within amino acids 200 - 250 of Caspase-1.
NCBI Gene ID #:
834
NCBI Official Name:
caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
NCBI Official Symbol:
CASP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_150634
Protein GI Number:
15431328
Purification:
Caspase-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
Depending on cell lines or tissues used, other cleavage products may be observed.
Swissprot #:
P29466
User NOte:
Optimal dilutions for each application to be determined by the researcher.