Chemodex

DAF-FM DA

Product Code:
 
CDX-A0024
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
Short term: +4°C. Long term: -20°C
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Chemical Structure
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Chemical Structure

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CodeSizePrice
CDX-A0024-M0011 mg£365.00
Quantity:
CDX-A0024-M0055 mg£1,428.00
Quantity:
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Further Information

Alternate Names/Synonyms:
4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate; Diaminofluorescein-FM diacetate
Appearance:
Light yellow powder.
CAS:
254109-22-3
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
InChiKey:
BEVHTVRRVVEMEF-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 254109-22-3. Formula: C25H18F2N2O7. MW: 496.42. Synthetic. DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument suitable for detecting fluorescein.
MDL:
MFCD16872050
Molecular Formula:
C25H18F2N2O7
Molecular Weight:
496.42
Package Type:
Vial
Product Description:
DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument suitable for detecting fluorescein.
Purity:
>98% (HPLC)
SMILES:
CNC1=C(N)C2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=C(F)C(OC(C)=O)=C3)C=C(OC(C)=O)C(F)=C2
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.

References

(1) J. S. Carroll et al.; Anal. Chem. 79(14), 5133 (2007) | (2) Y. Itoh et al.; Anal. Biochem. 287(2), 203 (2000) | (3) H. Kojima et al.; Angew. Chem. 111(219), 3419 (1999)