Abnova Corporation

Dispase (B. polymyxa)

Product Code:
P5318
Product Group:
Native Proteins
Regulatory Status:
RUO
Storage:
Store at 4°C.
Aliquot and store at -20°C after reconstitution with water or commonly used balanced salt solutions or media.
Aliquot to avoid repeated freezing and thawing.
 

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This product comes from: Taiwan.
Typical lead time: 14-21 working days.
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Further Information

Antigen Target Species:
Bacteria
Form:
Lyophilized
Preparation Method:
Native protein purified from Bacillus polymyxa.
Product Description:
Lyophilized. Partially purified. Animal Free/AF. Dispase (B. polymyxa) is a non-mammalian animal origin free (AOF) metallo, neutral protease. The mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.
Protein Accession:
P29148
Recommend Dilutions:
Neutral Protease (Dispase) Assay; Tissue disaggregation and subcultivation; Prevention of unwanted cell clumping; Preparation of cells for culture; Separation of intact epidermis from dermis and intact epithelial sheet in culture from the substratum; Harv
Specificity:
Non-specific cleavage of peptide bonds containing leucine and phenylalanine.
Tag:
None