ScienCell's GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Kit (GQH-TMH) is designed to facilitate gene expression profiling of 88 key genes involved in three types of hematological malignancy development (acute myeloid leukemia, precursor B lymphoblastic leukemia, and precursor T lymphoblastic leukemia), and is based on extensive literature research. Brief examples of how included genes may be grouped according to function and malignancy types are shown below:
- Cell cycle regulation: - CCNT1, CCNT2, CDK9, CDK14, CDKN2C
- Signaling mediation: - IGF1R, BMP2K, PROM1
- Differentiation regulation: - CSF1R, CD14, DUSP6, GZMB, HOXA9, CCR7, FUT8, SIX4, EYA1
Mutated transcription factors identified -
- in acute myeloid leukemia: - AML1, CEBPA, PLZF, ZBTB16, SPI1
- in precursor B lymphoblastic leukemia: - TCF3, PBX1
- in precursor T lymphoblastic leukemia: - TLX1, TLX3
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
This kit is designed to facilitate gene expression profiling of 88 key genes involved in three types of hematological malignancy development (acute myeloid leukemia, precursor B lymphoblastic leukemia, and precursor T lymphoblastic leukemia), and is based on extensive literature research.
