ScienCell's GeneQuery™ Human Diabetes, Type I (Juvenile, Insulin-dependent) qPCR Array Kit (GQH-DB1) is designed to facilitate gene expression profiling of 88 key genes involved in the development and progression of insulin-dependent diabetes mellitus. Type 1 diabetes mellitus is characterized by abnormally high blood sugar levels resultant of a disorder in glucose homeostasis. Brief examples of how included genes may be grouped according to function are shown below:
- Blood glucose regulation: - INS, INSR, SLC2A2, SLC30A8, ABCC8
- Autoimmunity: - GAD1, GAD2, LTA, HLA-A, OAS1
- Fat metabolism: - ADIPOQ, LPL, PON1, LEP, RETN
- Cholesterol regulation: - APOA1, APOB, LPA, CETP, AMBP
- Hormone regulation: - PREP, IGF1, SST, HGF, REN
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
This kit is designed to facilitate gene expression profiling of 88 key genes involved in the development and progression of insulin-dependent diabetes mellitus.
