ScienCell's GeneQuery™ Human Hepatitis B qPCR Array Kit (GQH-HPB) is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis B pathogenesis. Hepatitis B is a liver infectious disease caused by the Hepatitis B virus (HBV). The HBV infection of hepatocytes triggers immune response cascades to regulate HBV replication and hepatocyte survival. Brief examples of how included genes may be grouped are shown below:
- HBV binding factor: - HSPG2, CPD
Signaling pathways -
- MAPK: - MAPK1, MAP3K1, MAPK8, MAPK10, MAP2K1, RAF1, ELK1, YWHAB, YWHAQ, FOS
- PI3K/AKT: - AKT1, PIK3CA, PIK3CG, PIK3R1, PIK3R3, PIK3R5
- Toll-like receptor: - TLR2, TLR3, TLR4, IFNAs, IFNAR1, IFNB1
- JAK/STAT: - STAT1, STAT3, JAK1, MMP9, NFKB1, RELA, SRC
- Genes implicated in HBV infection: - LAMTOR5, RSF1, APOBEC3G, GPT, TNF, AIP, AFP, MBL2, F2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
This kit is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis B pathogenesis.
