ScienCell's GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation. HIF1 activity is a major component of the human response to hypoxia. Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:
- Cell cycling: - DHFR, MAP3K5, POLE2, PRC1, SKP2
- Extracellular matrix remodeling: - COL1A1, COL5A1, LOX, P4HA1, PLOD1
- Angiogenesis: - HMOX1, MMP2, VEGFA, ANGPTL4, PGF
- Metabolism: - PKM, STC2, DHX35, DDX11, ERO1A
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
This kit is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation.
