CD80 Antibody [10A1]

Product Code:
PSI-RF16042

Product Group:
Primary Antibodies

Supplier:
ProSci

Antibody Isotype:
IgG2b

Antibody Clonality:
Monoclonal

Antibody Clone:
10A1

Regulatory Status:
RUO

Target Species:
Human

Applications:

Flow Cytometry
Immunocytochemistry (ICC)
Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)

Product Type:
Immune Checkpoint

Storage:
CD80 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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Further Information

Additional Names:

CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1

Application Note:

CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL. Flow cytometry at 10 μg/ml.

Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Background:

CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).

Background References:

Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

Buffer:

CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

CD80 antibody was raised against the extracellular domain of human CD80.

NCBI Gene ID #:

941

NCBI Official Name:

CD80 molecule

NCBI Official Symbol:

CD80

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Predicted: 32 kDa

Observed: 50 kDa

Protein Accession #:

NP_005182

Protein GI Number:

4885123

Purification:

CD80 Antibody is supplied as protein A purified IgG2b.

Research Area:

Immunology

Swissprot #:

P33681

User NOte:

Optimal dilutions for each application to be determined by the researcher.

CD80 Antibody [10A1]

Further Information

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