ProSci

CD80 Antibody

Product Code:
 
PSI-8679
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
CD80 antibody can be stored at 4˚C for three months and -20˚C stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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<strong> Figure 1 WB Validation in Human Raji Cells </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong> Figure 2 WB Validation in Human Thymus </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000
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<strong>Figure 3 WB Validation in Human Tonsil </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 WB Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 WB Validation in Rat Thymus </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 6 Immunofluorescence Validation of CD80 in Human Tonsil</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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<strong>Figure 7 Immunofluorescence Validation of CD80 in Human Tonsil</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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<strong>Figure 8 Immunofluorescence Validation of CD80 in Mouse Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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<strong>Figure 9 Immunofluorescence Validation of CD80 in Rat Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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<strong> Figure 1 WB Validation in Human Raji Cells </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 2 WB Validation in Human Thymus </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000
<strong>Figure 3 WB Validation in Human Tonsil </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 WB Validation in Rat Thymus </strong><br> Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunofluorescence Validation of CD80 in Human Tonsil</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of CD80 in Human Tonsil</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 8 Immunofluorescence Validation of CD80 in Mouse Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 9 Immunofluorescence Validation of CD80 in Rat Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

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Further Information

Additional Names:
CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
Application Note:
WB: 1-4 μg/mL; IHC-P/IF: 5-20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in the human, mouse and rat samples. All other applications and species not yet tested.
Background:
CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Background References:
  • Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
  • Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.
Buffer:
CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (71%)
Immunogen:
CD80 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human CD80.

The immunogen is located within amino acids 60 - 110 of CD80.
NCBI Gene ID #:
941
NCBI Official Name:
CD80 molecule
NCBI Official Symbol:
CD80
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
Predicted: 60 kD

Observed: 60 kD
Protein Accession #:
NP_005182
Protein GI Number:
4885123
Purification:
CD80 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cell Cycle,Cancer,Immunology
Swissprot #:
P33681
User NOte:
Optimal dilutions for each application to be determined by the researcher.