ScienCell's GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit (GQH-BTD) is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells. Brief examples of how included genes may be grouped according to their functions are shown below:
- CLP markers: - MME, CD34, IL7R, FLT3
- ProB-PreB-immature B cell markers: - MME, MS4A1, SPN, PTPRC, IL7R, CD79A/B, CD40, CD19
- B1 cell markers: - CD19, MS4A1, CD27, SPN, CD40LG
- Regulatory B cell markers: - CD1D, CD5, CD19, CR2, CD24
- Marginal Zone B cell markers: - CD1C, CD19, MS4A1, CR2, CD24
- Follicular B cell markers: - CD19, MS4A1, CR2, CD22, FCER2
- Memory B cell markers: - CD27, CD80, CD84, CD86
- Plasmablast-plasma cell markers: - CD19, CD27, CD38, IL6R, SDC1, SLAMF7
- Double-negative and double-positive stage T cell markers: - CD1A, CD3D/E/G, CD4, CD5, CD7, CD8A/B, CD38, SELE/L/P, CD160, CD200
Note : - all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC). GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
This kit is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells.
