As these organoid models grow in complexity, the demand for products that can perform in dense 3D environments is critical. Our supplier, Leinco Technologies, offers antibodies, recombinant proteins, and tissue clearing technologies engineered to provide high penetration, low background, and physiological safety for tumour-immune co-cultures and spatial phenotyping.
Read on to discover Leinco’s featured solutions for organoid research!
Contents
- In vivo Functional Grade Abs
- Recombinant Biosimilars
- ADAPT-3D™ Tissue Clearing Kit
- Recombinant Proteins
In vivo Functional Grade Antibodies
Leinco Technologies provides industry-leading in vivo grade antibodies specifically designed for use in preclinical mouse model studies and other in vivo biological research applications. These high-performance antibodies are essential tools for exploring cell signalling pathways, immune response mechanisms, tumour microenvironments, and therapeutic efficacy in animal models.
Leinco’s in vivo antibodies are rigorously purified to meet the highest quality standards, featuring:
- Ultra-Low Endotoxin Levels (≤0.5 EU/mg) for Minimal in vivo Interference
- High Monomer Content (≥98%) with No Detectable Aggregates or Leachable Protein A
- Pathogen-Free Certified Antibodies (Validated via IDEXX Impact I PCR Screen)
- Functional Grade Purity Standards for Preclinical & in vivo Research
- Manufactured in an ISO 9001:2015 and ISO 13485:2016 certified facility
Leinco offers two antibody product lines tailored for in vivo use:
- In vivo GOLD™ Antibodies: High-purity reagents ideal for basic in vivo applications and mechanistic studies.
- In vivo PLATINUM™ Antibodies: Our top-tier antibodies, optimised for preclinical use, are verified to be pathogen-free, with low endotoxin levels and higher purity for sensitive in vivo research environments.
Recombinant Biosimilars
Research-grade biosimilar antibodies are produced by recombinant technology using the variable region sequences of therapeutic antibodies, resulting in the same specifications as approved biologics without the pharmaceutical prices.
Leinco’s biosimilar specifications:
- Purity > 95% by SDS-PAGE and ≥ 98% monomer by analytical SEC
- Endotoxin ≤ 0.5 EU/mg as determined by the LAL method
- Manufactured using protein-free, animal-origin-free cell culture media
- Ultra-low levels of leached protein A
- Sterile filtered and formulated in Leinco’s in vivo grade PBS pH 7.2 containing no K or Ca, additives, preservatives, or carrier proteins
- Many applications – including FC, ELISA, IHC, FA, PK, potency assays and IVD
- Pathogen tested (IMPACT1)
ADAPT-3D™ Tissue Clearing Kit
ADAPT-3D is a standardised, high-throughput protocol designed to solve the primary challenge of large-volume imaging: deep-tissue probe penetration.
ADAPT-3D (Accelerated Deep Adaptable Processing of Tissue for 3-Dimensional Imaging) has an adaptable and versatile nature for use with a wide range of tissues and model systems. This technology is designed for microscopy to prepare biological tissues for 3D fluorescence imaging. The ADAPT-3D method involves a protocol that serves as a guide with recommended incubation times for fixation, decolourisation, delipidation, and refractive index matching (RIM). The ADAPT-3D method was developed with inspiration from advances in aqueous-based clearing methods to achieve rapid, aqueous 3D imaging.
All ADAPT-3D components are available for individual purchase. This modular approach allows you to scale efficiently by ordering only the specific elements your project requires. Alternatively, you can purchase them together in the ADAPT-3D Tissue Clearing Kit.
The ADAPT-3D Tissue Clearing Kit is used to prepare fixed tissue samples for 3-dimensional fluorescence tissue imaging. This allows for improved light penetration in 3D tissues, enhancing the detection of fluorescent signals deeper within the samples. Experience faster clearing, less shrinkage and exceptional preservation of fluorescence and tissue morphology.
Specific uses highlighted in the sources include:
- Imaging human intestinal tissue after fixation, decolourisation, delipidation, and antibody staining.
- Imaging intact mouse skull and brain to detect specialised channels between the skull and underlying tissue without meningeal tearing.
- Whole-organ imaging, such as mouse brain, spleen, lung, and liver, with retention of endogenous fluorescent reporters.
- Light-sheet microscopy of cleared whole mouse brains with strong retention of fluorescent intensities of endogenous reporter proteins like eYFP and tdTomato.
- Imaging bone with adjacent soft tissues like the skull-brain interface after decalcification.
- Deep immunolabeling and detection of antigens such as tight junctional proteins, smooth muscle actin, S100A9, CD163, and IBA1 in various tissues, like leptomeninges and intestine.
- Compatible with multiple imaging modalities, including widefield, confocal, and light-sheet microscopy.
The ADAPT-3D Kit adheres to both ISO 9001:2015 and ISO 13485:2016 manufacturing standards, ensuring the highest consistency and performance.
Recombinant Proteins
Leinco Technologies provides recombinant proteins produced using mammalian expression systems, with Chinese Hamster Ovary (CHO) or Human Embryonic Kidney (HEK) cells as the host to achieve the highest level of biological activity.
Leinco’s Protein Specifications:
- >95% Purity for most proteins
- Longer storage and stability
- Bulk proteins re-assayed to assure high biological activity
- All proteins are Carrier Free/Low Endotoxin
Information provided by Leinco Technologies.
Caltag is the distributor of Leinco Technologies’ products in the UK and Ireland. If you have any questions about these products, please contact us.